Microtome

The rotary microtome is the most common instrument found in a histology laboratory. Although most microtomes are manual, some are automatic or semi-automatic. In order for tissue to be viewed under the microscope it must be thin enough to allow light to pass through and be mounted on a glass slide. A microtome is a mechanical device which helps to make thin sections of paraffin wax embedded tissues using a very sharp blade. care must be taken when handling blades or cutting section. 

1. Microtome base plate or stage: A platform which has rails that secure the knife holder base.
2. Knife holder base: A part that anchors the knife holder to the microtome stage. The knife holder base can be moved toward or away from the block, but MUST be stationary and locked during microtomy.
3. Knife holder: This part is comprised of several components including the blade clamp that holds the blade, the knife tilt for adjusting the knife angle, and the face plate that guides that ribbons away from the blade and towards the operator.
4. Cassette clamp or block holder: Holds the paraffin block in place. Typically, the block moves up and down with each revolution while the blade is stationary. The block holder may have knobs that allow the user to manipulate the block face in various directions to bring the tissue in alignment with the blade.
5. Coarse handwheel: Moves the block holder either toward the knife or away from the knife.
6. Advancement handwheel: Turns in one direction and advances the block toward the knife at the specified microns. Most handwheels are equipped with a safety lock to prevent the wheel from releasing and having the block holder come down towards the blade while a block is inserted or removed. The safety lock should be used anytime the microtomist is not actively sectioning paraffin blocks.
7. Micron adjustment: Micron settings for section thickness can range from 1 to 60 microns on most microtomes.